MAPK信号通路在炔雌醚诱导大鼠生精细胞凋亡中的作用研究

doi:10.11843/j.issn.0366-6964.2016.02.023

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (2): 381-387.doi: 10.11843/j.issn.0366-6964.2016.02.023

MAPK信号通路在炔雌醚诱导大鼠生精细胞凋亡中的作用研究

李健^1*，陈耀星²，陈福宁³，王子旭²，曹静²，董玉兰²

（1.河南科技大学动物科技学院，洛阳 471003； 2.中国农业大学动物医学院，北京 100193；3.北京昌平区中西医结合医院，北京 102208）

收稿日期:2015-09-08 出版日期:2016-02-23 发布日期:2016-02-23
通讯作者: 李健，E-mail：lijian800702@126.com
作者简介:李健(1980-)，男，安徽亳州人，博士，副教授，主要从事生殖毒理学、动物解剖学与组织胚胎学教学与研究
基金资助:
河南省教育厅高等学校重点科研项目基础研究计划（16A230004）；河南科技大学青年科学基金项目（2015QN032）；国家级大学生创新创业训练计划项目（201310464065）

The Role of MAPK Signaling Pathway in Quinestrol-induced Apoptosis of Spermatogenic Cells in Rat

LI Jian^1* ，CHEN Yao-xing²，CHEN Fu-ning³，WANG Zi-xu²，CAO Jing²，DONG Yu-lan²

(1.College of Animal Science and Technology，Henan University of Science and Technology，Luoyang 471003， China；2.Laboratory of Veterinary Anatomy，College of Veterinary Medicine，China Agricultural University，Beijing 100193，China；3.Changping Hospital of Integrated Chinese and Western Medicine，Changping，Beijing 102208，China)

Received:2015-09-08 Online:2016-02-23 Published:2016-02-23

摘要/Abstract

摘要：

旨在探讨MAPK信号通路在炔雌醚诱导大鼠生精细胞凋亡中的作用。将20只8周龄雄性SD大鼠随机分为4组，适应饲养1周后，按体重分别以0.00、0.01、0.10、1.00 mg•kg^-1腹腔注射溶解于橄榄油的炔雌醚，50 μL•只^-1，每天1次，连续1周。处理结束后，取睾丸并制备组织切片，通过HE染色观察睾丸组织结构的变化；通过免疫组织化学方法检测睾丸生精细胞PCNA、MAPK信号通路磷酸化ERK（p-ERK）、磷酸化JNK（p-JNK）及磷酸化P38（p-P38）蛋白表达，TUNEL法检测细胞凋亡。结果显示，炔雌醚处理后大鼠睾丸曲细精管上皮厚度下降，各级生精细胞数量显著减少，细胞皱缩，间隙增大，结构松散，管腔内成熟精子数量减少。生精细胞PCNA与p-ERK蛋白表达显著减少；TUNEL、p-JNK蛋白及p-P38蛋白表达则显著增加。综上表明，炔雌醚暴露通过影响MAPK信号传导通路抑制生精细胞增殖，促进细胞凋亡，最终抑制大鼠睾丸发育。

Abstract:

This experiment was conducted to study the role of MAPK signaling pathway in quinestrol-induced apoptosis of spermatogenic cells in rat.Twenty 8-week-old male SD rats were randomly divided into 4 groups and were treated by using daily intraperitoneal injection of 0.00，0.01，0.10 and 1.00 mg•kg^-1 of BW quinestrol dissolved in olive oil for 1 week after 1-week adaptive feeding.At the end of the treatment，the testis tissue sections were prepared and the morphology of the testis tissue was observed by HE staining.The immunohistochemistry staining was performed to measure the expression levels of PCNA，MAPK signaling pathway of p-ERK，p-JNK and p-P38 in spermatogenic cells and TUNEL method was used to detect apoptosis of spermatogenic cells.The results showed that the epithelial thickness of rat seminiferous tube decreased as well as the number of spermatogenic cells decreased.Numerous spermatocytes and spermatids showed swelling or shrinkage and meanwhile the number of mature sperm reduced in the lumen after quinestrol exposure.The expressions of PCNA and p-ERK in the spermatogenic cells were significantly decreased.In contrast，the expressions of TUNEL，p-JNK protein and p-P38 protein were significantly increased.The results suggest that quinestrol exposure inhibited the development of rat testis by inhibiting proliferation and promoting MAPK-mediated apoptosis of spermatogenic cells.

中图分类号:

S865.1⁺2.3

李健，陈耀星，陈福宁，王子旭，曹静，董玉兰. MAPK信号通路在炔雌醚诱导大鼠生精细胞凋亡中的作用研究[J]. 畜牧兽医学报, 2016, 47(2): 381-387.

LI Jian，CHEN Yao-xing，CHEN Fu-ning，WANG Zi-xu，CAO Jing，DONG Yu-lan. The Role of MAPK Signaling Pathway in Quinestrol-induced Apoptosis of Spermatogenic Cells in Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 381-387.

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MAPK信号通路在炔雌醚诱导大鼠生精细胞凋亡中的作用研究

The Role of MAPK Signaling Pathway in Quinestrol-induced Apoptosis of Spermatogenic Cells in Rat

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